LiquidScan®

ISOLATE RARE CELLS AND EXOSOMES – CTCs, TROPHOBLASTS, EVs

BioFluidica has created LiquidScan® as a unique, fully-automated system for isolating and enriching rare cells and exosomes from liquid biopsy samples. The system uses a microfluidic chip surfaced with antibodies targeting biomarkers of interest, creating the future of liquid biopsy.

*LiquidScan is for Research Use Only

LIQUIDSCAN WORKFLOW

1

LOAD

Flow whole blood over capture chip

2

CAPTURE

Capture rare cells or exo-somesp

3

WASH

Wash to eliminate background

4

ELUTE

Elute rare cells or exosomes

5

ANALYZE

1 The BioFluidica LiquidScan hardware & software modules are integrated into the Hamilton Microlab STAR or STARlet liquid handling robot.

2 LiquidScan Software automates loading the sample(s) onto the microfluidic chips, washing to remove background cells and cleavage and elution of captured cells or EVs from the chip.

3 Our custom software creates a secure-loop system, two-pipette tips per chip, which dispense and aspirate simultaneously enabling relatively large volumes of liquid biopsy sample to be passed through the chip.

THE LIQUIDSCAN SYSTEM

  • LiquidScan Microfluidic Chip Processing Module
  • LiquidScan Reagent Kit
    (with LiquidScan Microfluidic Chips surfaced with antibodies)
  • LiquidScan Software
  • LiquidScan installs on a Hamilton Microlab STARlet or STAR liquid handling robot
    (Contact us regarding pre-owned, new or adding LiquidScan to one you already have)

*For Research Use Only.

LIQUIDSCAN REAGENT KITS

  • Reagent plate with contents ready to process 4 or 8 microfluidic chips
  • Microfluidic chips (4 or 8):
    • Antibody-surfaced sinusoidal chips for rare-cell capture or,
    • Antibody-surfaced pillar chips for exosome subpopulation capture
  • Sample tubes (8)
  • Waste tubes or re-process sample tubes (8)

PILLAR & SINUSOIDAL CHIPS

  • Microfluidic chip surface is tunable to isolate biomarkers of interest. Established for isolation of Exosomes in plasma and Rare cells in whole blood.
  • Release of isolated target analytes can be optimized for downstream applications.
  • Enzymatic release for evaluation of EV or Rare Cell surface markers or lyse on chip for nucleic acid or protein investigation.

VERSATILE PLATFORM

LiquidScan Processing Module holds up to eight BioFluidica Microfluidic Chips and has been designed to provide a walk-away automated system for the enrichment of rare cells and exosomes from liquid biopsy samples, including whole blood, plasma, urine, and saliva. LiquidScan increases the relative population of rare cells and specific subtypes of exosomes above the Limit-of-Detection for most molecular analysis methods, including NGS, RNA-Seq, Agena, ddPCR, and qPCR.

The LiquidScan system is offered for sale for installation in your facility, or, BioFluidica offers Services, where we do the work in our Core Labs on a fee-for-service basis. We can perform complete studies or run Pilot Projects. We will discuss the project with you and develop a statement of work with pricing.

The technology is flexible and accommodates various processing protocols. Services include surfacing the channels of microfluidic chips with antibodies sourced by you or we can source for you.

Services include the following and can be extended to isolate most biomarkers or infectious agents of interest: