NEXT-GENERATION LIQUID BIOPSY

BioFluidica has created the next-generation liquid biopsy with the creation of LiquidScan^®. LiquidScan isolates rare biomarkers present in liquid biopsy samples and works based on an antibody's affinity and specificity for its complementary epitope/target. The channels of a microfluidic device are surfaced with an antibody(s) against the desired target within a liquid biopsy sample, for example a circulating tumor cell (CTC) or an exosome subtype. The antibodies in the channels are tethered to the chip by linkers that allow for either photoactivated or enzymatic cleavage of the linker. A liquid biopsy sample is passed through the chip, allowing affinity capture of the desired biomarker. Following sample loading, the chips are washed to remove unwanted cells (background). The chips are then bathed in 415 nm light or treated with a heat-activated enzyme to cleave the linker and release the isolated analytes of interest. Analytes are then eluted and collected, ready for downstream analysis. The process results in enrichment over the background of captured biomarker, e.g., an eluate would have >10 CTCs in a total cell count of 100.